Distance Determination in Proteins by EPR

Interspin Distance Determination

Precise measurement of electron-electron interspin distances in the range 20-80 Å is becoming more and more important, e.g. in cardiology research.

: Data from H. Hara, BrukerBiospin, K.K. Arata & T. Arata, Osaka Univ., Japan

With our ELEXSYS E580Q as well as our E680 system and the ELDOR upgrade option, DEER distance measurement experiments are now commonly employed at Q-band and W-band. The increase in frequency provides a clear increase in sensitivity in comparison to X-band DEER measurements.
ELEXSYS systems

Human cardiac Troponin C

Positions labelled with nitroxides by site-directed spin labelling
Single crystal not required
No limit in molecular weight
Measuring time typ. 60 min
High accuracy: 26 ± 0.2 Å